Supplementary data: The molecular evolution of cancer associated genes in mammals

Cancer is a disease that many multicellular organisms have faced for millions of years, and species have evolved various tumour suppression mechanisms to control oncogenesis. Although cancer occurs across the tree of life, cancer related mortality risks vary across mammalian orders, with Carnivorans particularly affected. Evolutionary theory predicts different selection pressures on genes associated with cancer progression and suppression, including oncogenes, tumour suppressor genes and immune genes. Therefore, we investigated the evolutionary history of cancer associated gene sequences across 384 mammalian taxa, to detect signatures of selection across categories of oncogenes (GRB2, FGL2 and CDC42), tumour suppressors (LITAF, Casp8 and BRCA2) and immune genes (IL2, CD274 and B2M). This approach allowed us to conduct a fine scale analysis of gene wide and site-specific signatures of selection across mammalian lineages under the lens of cancer susceptibility. Phylogenetic analyses revea..., For information on sampling and analysis see: https://doi.org/10.1038/s41598-024-62425-0, , # Supplementary data: The molecular evolution of cancer associated genes in mammals

\[Access this dataset on Dryad](Dataset DOI link)

The dataset contains supplementary files for the comparative genomic analysis conducted in our study. Analysis was conducted on 9 cancer associated genes across 868 mammalian species. Data files include sequence alignments, phylogenetic trees and values generated from analyses conducted on the data monkey webserver ([https://www.datamonkey.org/](https://www.datamonkey.org/)): MEME and FUBAR.

Genes: Based on their roles associated with cancer development and progression, nine genes were selected for in depth analyses. Three oncogenes; Growth factor receptor-bound protein 2 (GRB2), Fibrinogen like protein 2 (FGL2) and Cell Division Control protein 42 homolog (CDC42) and three tumour suppressors; Lipopolysaccharide Induced Tumour Necrosis Factor (LITAF), Caspase 8 (Casp8) and Breast Cancer gene 2 (BRCA2), and three immune genes; Interleukin-2 (IL2), Clust...,