The role of TEFM in mitochondrial gene expression [smallRNA]

We investigated the role of TEFM in vivo by creating heart- and skeletal-muscle specific knockout of this gene. We identified that TEFM is required for the processivity of POLRMT and its loss leads to significantly decreased mitochondrial transcription. Small RNA from isolated heart mitochondria from four control mice (L/L) and four Tefm knockout mice (L/L, cre), was sequenced and analysed for differential expression.我们通过构建线粒体转录延伸因子（TEFM）基因的心脏及骨骼肌特异性敲除模型，在体内探究了该基因的生物学功能。研究证实，TEFM是线粒体RNA聚合酶（POLRMT）的持续合成活性所必需的，其缺失会导致线粒体转录水平显著下调。我们对4只对照小鼠（L/L基因型）与4只Tefm敲除小鼠（L/L, cre基因型）分离得到的心脏线粒体中的小RNA进行了测序，并分析其差异表达情况。