pEC50 values and fold change in EC50 for AVP-induced cAMP and IP1 accumulation.

Transfected HEK293FT or COS7 cells were treated as per Figure 1 or 2. Data shown are the pEC50 values for AVP treatment (30 min) and are the mean ± SEM of 3 or 5 independent experiments for cAMP and IP1 respectively. Fold change from the wild-type receptor EC50 data are given in adjacent columns.*, significantly different to the corresponding wild-type receptor in the same cell line (P**, significantly different to M311V in the same cell line (P***, significantly different to R181C HA-V2R in HEK293FT cells. ND = Not determined.