Transcription profiling of mouse olfactory epithelium from Harlequin mutants compared to littermate controls

Microarray analysis of gene expression in the olfactory epithelium of Harlequin mouse as a model of oxidative-stress induced neurodegeneration of olfactory sensory neurons Experiment Overall Design: Olfactory epithelium from Harlequin mutant mice and littermate control mice was microdissected for RNA extraction and hybridization on Affymetrix microarrays. We compared levels of gene expression in 6-month old mice to begin to identify mechanisms of oxidative-stress induced neurodegeneration and to correlate the cellular changes that we observed in the olfactory epithelium by using histology and immunohistochemistry with gene expression changes.以小丑鼠（Harlequin mouse）作为嗅觉感觉神经元氧化应激诱导退行性变模型的嗅上皮基因表达微阵列分析 实验整体设计：从小丑突变型小鼠及其同窝对照小鼠中显微解剖嗅上皮，用于RNA提取并在Affymetrix微阵列上进行杂交。本研究通过比较6月龄小鼠的基因表达水平，以初步阐明氧化应激诱导神经元退行性变的相关机制，并将我们通过组织学与免疫组织化学方法在嗅上皮中观察到的细胞变化与基因表达改变进行关联分析。