CI expression shown by average larval deposition rates in crosses between GmmApo females mated with GmmWt males.

Larval deposition rates for each gonotrophic cycle and each cross type replicate were determined by dividing the number of larvae deposited per day by the number of remaining females in the cage on the day of larviposition, and summing the values for each gonotrophic cycle. Mean deposition rate values with different superscripted letters are statistically different from each other (Pn was calculated by combining the total number of females alive when the first larva were deposited for the three replicates of each cross type. GmmWt = Wild-type flies with all three symbionts, GmmApo = flies treated with tetracycline that lack Wigglesworthia, Sodalis, and Wolbachia, and GmmWig− = flies treated with ampicillin that lack only Wigglesworthia.