Whole genome sequencing of strain PAMC 20958 and one type strain of genus Halocynthiibacter.

A sediment sample collected from the coast of Svalbard, Arctic (78° 55' N, 11° 53' E). The sediment sample was suspended in 20 % glycerol and preserved at -80C until use. Strain PAMC 20958T was isolated by using standard dilution plating method on ZoBell agar containing 15 g bacto agar, 5 g bacto peptone, 1 g yeast extract, and 0.1 g ferric citrate in 1 L of 0.2-μm filtered seawater and incubating the plate at 10 C for 12 days. After determination of the optimum growth temperature, strain PAMC 20958T was maintained routinely on Marine agar (MA; BD Difco) or in marine broth (MB; BD Difco) at 20 °C and preserved as glycerol suspensions (20 % in distilled water, v/v) at -80 °C. Reference strain H. namhaensis KCTC 32362T was obtained from the KCTC (Korean Collection for Type Cultures, Korea) and was maintained routinely on MA at 20 °C.

Genome relatedness between PAMC 20958T and H. namhaensis KCTC 32362T was investigated by whole genome sequencing.
-Analysis of Bacterial Community in coast of Svalbard, Arctic.
-Basic data acquisition of PAMC(Polar and Alpine Microbial Collection).
-Academic report of novel strain.