3D fluorescence dataset of tissue cleared pig cochlea

Tissue clearing and light-sheet fluorescence microscopy were applied for
3D profiling of intact cochleae. However, the spiral ganglion neurons
(SGNs) remain relatively understudied compared to hair cells and
supporting cells, especially in large animal models. Here we: 1)
introduced collagenase treatment to the current protocol of tissue
clearing to enhance uniform antibody staining of SGNs within the pig
cochlea, and 2) adopted a deep learning object detection model to locate
and count SGNs in large 3D datasets via Spiner (Spiral ganglion neuron
profiler). This is an example pig cochlea dataset for running the designed
Spiner workflow. The dataset is structured by TeraStitcher
compatible two-level hierarchy of folders, and the TIFF images can be
opened with image tools (e.g., ImageJ) that support the TIFF
format.