Somatic ACE regulates self-renewal of mouse spermatogonial stem cell via MAPK signaling pathway

To further explore the mechanism involved in sACE mediated SSCs proliferation. We established microarray gene expression profiles with or without captopril treatment in cultured SSCs and identified 651 differentially expressed genes (Fold Change>2, P<0.05). Among them, 297 genes were remarkably upregulated and 354 genes were downregulated in three comparison groups after captopril treatment.Then bioinformatical analysis including GO analysis and KEGG pathway analysis were performed and both of them indicated these differentially expressed genes might be involved in regulation of tyrosine phosphorylation of STAT protein, ERK1 and ERK2 cascade, MAPK signaling, Jak-STAT signaling and et al. Four MAPK signaling related genes (Pla2g12b, Cacna2d1, Dusp2, Fgf3) from this signature was quantified by real-time PCR. Captopril can inhibite the activity of sACE, so 0.01M captopril was added to SSCs culture for 24 hours. SSCs cultured with 0.01M ddH2O served as Negative control. Three independent experiments were performed .