Mechanical cues control mutant p53 stability through a Mevalonate/RhoA axis

To investigate the genes differentially expressed upon plating on top of matrixes with different stiffness, we compared the expression profiles of MDA-MB-231 breast cancer cells plated on a stiff substrate (plastic) with the same cells plated on a soft substrate (hydrogels 0.7 kPa). Keywords: expression profiling by array We collected RNA from MDA-MB-231 cells plated and cultured from 2 days on a stiff substrate and on a soft substrate. Samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each condition for a total of 8 samples.