Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapies. Cellular crosstalk regulates the aqueous humor outflow pathway and provides new targets for glaucoma therapies

Angiopoietin-1 signaling is essential for development of Schlemm's canal, a large lymphatic-like endothelial vessel in the iridocorneal angle. Single cell RNA sequencing is a powerful tool allowing for examination of the specific transcriptome of each cell type within a tissue. Here, we used single cell RNA sequencing to examin the structures of the mouse iridocorneal angle, using loss of Schlemm's canal in neural-crest specific Angpt1 knockout mice to aid in identification of Schlemm's canal endothelial cells. This dataset provides a resource for analysis of trabecular meshwork / Schlemm's canal crosstalk and the role(s) of Angpt1 in formation of the iridocorneal angle. Overall design: The 10x Genomics Chromium platform was used to obtain single cell transcriptomes from iridocorneal angle region of 6 week old wild-type and neural crest-specific Angpt1 knockout mice.