Comparison of DNA methyltransferase-1 (Dnmt1) deleted urogenital sinus epithelium compared to wildtype urogenital sinus epithelium

Dnmt1 is an important regulator of tissue development and differentiation. To assess the effects of epithelium Dnmt1 deletion in the developing urogenital sinus (precursor of the urethra and prostate in males), we isolated urogenital sinus epithelial tissue from Dnmt1 deleted mouse embryos and wildtype mouse embryos. The transcriptomes were analyzed by RNA-seq A Shh cre driver was used to achieve Dnmt1 deletion in the epithelium of the developing urogenital sinus. Epithelium of the urogenital sinus of embryonic day 15.5 mouse embryos were isolated by trypsin digestion. Tissues from Dnmt1 mutants (Dnmt1cKO) were compared to wildtype embryos (Control) in this experiment.DNA甲基转移酶1（Dnmt1）是组织发育与分化的重要调控因子。为评估发育中的泌尿生殖窦（男性尿道与前列腺的前体）上皮组织的Dnmt1敲除效应，我们从Dnmt1敲除小鼠胚胎与野生型小鼠胚胎中分离了泌尿生殖窦上皮组织。采用RNA测序（RNA-seq）技术对转录组进行分析。借助Shh-Cre驱动工具，实现发育中泌尿生殖窦上皮组织的Dnmt1敲除。通过胰酶消化法，分离得到胚胎发育至第15.5天的小鼠泌尿生殖窦上皮组织。本实验中，将Dnmt1突变体（Dnmt1cKO）的组织与野生型胚胎对照组（Control）进行对比分析。