Dataset in support of the Southampton doctoral thesis 'Studies on plasmid-based genetic tools for C. muridarum: progress towards a replicating vector for transposon mutagenesis'

This dataset contains two databases demonstrating the raw data collected for measuring plasmid stabilty in C. muridarum strains. The first dataset contains data for quantifying the frequency of plasmid loss as a proxy for the rate of plasmid loss, by quantifying the proportion of plasmid-free inclusions in the total population identified by the absence of GFP expressed in trans. The second dataset contains qPCR data measured from samples of total genomic DNA extracted from harvested inocula at passage 3 and 6 only, for determination of plasmid copy number (absolute plasmid copies per chromosome). The qPCR protocol used primer pairs to target single copy genes located on the chromosome (omcB) and on the plasmid (CDS2).