Aconitate decarboxylase 1 regulates inflammation in colitis and colitis-associated carcinoma and maintains gut microbiome

Background: Aconitate decarboxylase 1 (ACOD1) is implicated in innate immunity and inflammatory responses. We determined the role of ACOD1 in colon inflammation and colitis-associated carcinoma (CAC). Methods: Human inflammatory bowel disease transcriptomic datasets and banked RNA samples were interrogated. C57 wild-type (WT) and Acod1 deletion mice were infected with Citrobacter rodentium as models of colitis. Tissues and stool were used for metabolomic and 16S microbiome analyses, respectively.Results: 16S microbiome analysis revealed significant differences in phyla and genera; notably an increase in Bacteroidetes and decrease in Proteobacteria in Acod1 deletion mice, indicating a dysbiotic state.