Impact of in ovo administration of xylo-and mannooligosaccharides on broiler chicken gut health

The aim of this study was to modify the host gut microbiota by in ovo stimulation on d12 of egg incubation with xylo-oligosaccharide and manno-oligosaccharide prebiotics,and to determine the changes occurring in intestines (ileum and caecum) in broiler chickens after stimulation.Hatching eggs from the parent flock of Ross308 broiler hens were purchased from a commercial hatchery, the total number of eggs was700. On d7, the eggs were candled (ovoscope, Fermo, Piotrow, Poland), and 300 fertilized eggs were chosen for the experiment. On d12 of incubation, the eggs were randomly divided into 5 groups (60 eggs in each group) and injected into the air chamber with 0.2mL of saline (0.2mmol/L)(control) or with one of the prebiotic solutions: xylotriose (XOS3), xylotetrose (XOS4), mannotriose (MOS3), or mannotetrose(MOS4).