Early gene expression differences in inbred mice predict susceptibility to spontaneous pulmonary adenomas

We examined if gene expression profiles differed between healthy (tumor-free) mice of strains that are known to have different pulmonary adenoma susceptibility (severity and frequency). Three females of 11 week old mice of 9 (129S1/SvlmJ, A/J, BALB/cJ, C3H/HeJ, C57BL/6J, C57L/J, DBA/2J, KK/HlJ, and MRL/MpJ) strains were selected for RNA extraction and hybridization on GeneChip® Mouse Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA). Each strain was compared to all other strains (36 unique comparisons total) to identify the most common differentially expressed probe sets among the population. The most common probe sets were then compared between strain groups (increased vs. decreased spontaneous pulmonary adenoma frequency and severity).本研究旨在探究已知肺腺瘤易感性（严重程度与发生频率）存在差异的品系的健康（无肿瘤）小鼠之间，基因表达谱是否存在差异。选取9个品系（129S1/SvlmJ、A/J、BALB/cJ、C3H/HeJ、C57BL/6J、C57L/J、DBA/2J、KK/HlJ及MRL/MpJ）的11周龄雌性小鼠各3只，进行RNA提取，并在GeneChip®小鼠基因1.0 ST阵列（GeneChip® Mouse Gene 1.0 ST Arrays）（Affymetrix，美国加利福尼亚州圣克拉拉）上完成杂交实验。将每个品系与其余所有品系进行两两比较（总计36组独特的对照组合），以鉴定群体中最常见的差异表达探针集。随后将上述最常见的探针集按照品系组（自发性肺腺瘤发生频率与严重程度升高组 vs. 降低组）进行分组比较。