Metadata supporting data files for the published article: Evaluation of Ductal Carcinoma In Situ Grade via Triple-Modal Molecular Imaging of B7-H3 Expression

<b>Summary</b>The data described in this metadata record underlie the figures in the related manuscript, and consist of tabulation of H-scores from immunohistochemical staining in 123 human specimens including benign epithelium ductal carcinoma in situ (DCIS), targeted enhancement (a.u.) values from molecular imaging of B7-H3 targeted microbubbles to differentiate normal glands from those harboring DCIS in a murine model, and photoacoustic and fluorescence molecular imaging intensities (a.u. and radiant efficiency respectively) with an anti-B7-H3 antibody-indocyanine green conjugate.<br>The related study aimed to perform an evaluation of DCIS grade via triple-modal molecular imaging of B7-H3 expression, as molecular imaging of B7-H3 expression may allow for active surveillance of DCIS. <br><b>Data access</b>The data underlying each figure, and their respective access conditions, are as follows.- Figure 2a:Human DCIS B7-H3 1A.ndpi, Human DCIS H&amp;E 1A.ndpi (coregistered B7-H3 and H&amp;E stained histology images x 6).Not publicly available in order to protect patient privacy.- Figure 2b, 2c:Human B7-H3 DCIS Histology Scoring.xlsx, Human B7-H3 DCIS Histology Scoring.pzfx.Included with this metadata record.- Figure 3a:mouse_DCIS_CD276-0029_c0.tif, mouse_DCIS_CD276-0029_c1.tif, mouse_DCIS_CD276-0029_c2.tif (3 .tif images for each mouse gland x 12 glands)Available upon request.- Figure 3b, 3c:Murine B7-H3 DCIS QIF.pzfxAll Murine B7-H3 Imaging Summary Data.pzfxIncluded with this metadata record.- Figure 4a.<br>m82_1R_CD276_Iseq.ANIMG, m82_1R_CD276_Iseq.BIMG m82_1R_CD276_Iseq.MXML, m82_1R_CD276_Iseq.PIMG, m82_1R_CD276_Iseq.PNG, m82_1R_CD276_Iseq.DCM, m82_1R_CD276_Iseq.VXML (7 files uniquely named for each of the 100 murine glands imaged)Available upon request--will require instruction to use.- Figure 4b, 4c:All Murine B7-H3 Imaging Summary Data.pzfxIncluded with this metadata record.- Figure 5a:2014-09-02-15-56-01_CD276 MS2 L1-2014-08-29-15-38-10_1.raw.bmode, 2014-09-02-15-56-01_CD276 MS2 L1-2014-08-29-15-38-10_1.raw.event, 2014-09-02-15-56-01_CD276 MS2 L1-2014-08-29-15-38-10_1.raw.pamode, 2014-09-02-15-56-01_CD276 MS2 L1-2014-08-29-15-38-10_1.raw.physio, 2014-09-02-15-56-01_CD276 MS2 L1-2014-08-29-15-38-10_1.raw (5 unique files for each of 70 murine glands imaged with identifying names)Available upon request--will require instruction to use.- Figure 5b, 5cAll Murine B7-H3 Imaging Summary Data.pzfxIncluded with this metadata record.<br>- Figure 6a:"KEW20140911152427_SEQ" (appended with 64 various file endings (.tif, .txt., png.,) for each of the 72 glands with unique identifying file names)Available upon request--will require instruction to use.- Figure 6b, 6c:All Murine B7-H3 Imaging Summary Data.pzfxIncluded with this metadata record.<br><br>Proprietary software is required for some of the files. These software include: Vevo LAB,Version 1.7.2.7310, VisualSonics, MATLAB, Living Image Software 4.0 (PerkinElmer), Prism GraphPad (Version 8.1.2), ImageJ (version 1.46r).<br>For all data requests please contact:Katheryne E. Wilson, Ph.D.Department of Radiology and Molecular Imaging Program at StanfordSchool of Medicine, Stanford University3155 Porter DrivePalo Alto, CA 94304P: 650-497- 5547Email: [e-mail]<br><b>Institutional Review Board or ethics committee</b>Human breast tissue samples were obtained retrospectively and were selected under an HIPAA compliant, Institutional Review Board-approved protocol at the University of California San Francisco.All experiments involving animals were approved by the Institutional Administrative Panel on Laboratory Animal Care at Stanford University.