mRNA expression changes upon microRNA downmodulation in colorectal cancer cell lines. Homo sapiens

Colorectal cancer cell lines were transduced with lentiviral antisense miRNA inhibitors (miRZIPs) targeting miR-429, miR-200b, miR-194 and miR-203. Global mRNA expression profiles were generated with Illumina arrays on transduced and control cells to identify mRNAs whose expression is regulated by the above microRNAs. Overall design: Colorectal cancer cell lines transduced with control vectors or with microRNA targeting vectors were profiled, together with wild-type cells, using two independent RNA extractions.