7F_S7CD_Luciferase assay_let-7 and lin-46 overexpression in lin-28::large_tag background

Luciferase assay data used to quantify the duration of larval stages of animals of the indicated genotypes. The file contains three sheets: Raw data: Contains the raw values measured by the luminometer (Berthold Technologies, Centro XS3 LB 960). Luminescence was measured every 10 min for 0.5 seconds. The luminometer was placed in a temperature-controlled incubator set to 18.5°C, which translated to an effective temperature of 22.5°C experienced by the animals inside the luminometer. Plate design: Position of animals of indicated genotypes in the 384-well luminometer plate. Annotated data: List of animals of indicated genotypes with the times of hatch, molt entry and exit and calculated durations of molt, intermolts and larval stages in hours. Animals excluded from the analysis are indicated by "0" in the "is_valid" column. wild type HW1993 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28(0) HW2706 lin-28(xe192) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV let-7(++) HW3771 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; xeSi287[let-7p::let-7] V lin-46(++) HW3530 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; lin-46(xe370) V let-7(++) lin-46(++) HW3809 xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; xeSi287[let-7p::let-7] lin-46(xe370) V lin-28::large_tag HW3226 lin-28(xe197) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV lin-28::large_tag; let-7(++) HW3757 lin-28(xe197) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; xeSi287[let-7p::let-7] V lin-28::large_tag; lin-46(++) HW3684 lin-28(xe197) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; lin-46(xe370) V lin-28::large_tag; let-7(++) lin-46(++) HW3882 lin-28(xe197) I; xeSi312[eft-3p::luc::gfp::unc-54 3'UTR, unc-119(+)] IV; xeSi287[let-7p::let-7] lin-46(xe370) V