Physiochemical Properties of WRKY Transcription Factor Proteins of Grapes.

Although the function of a TF is determined by its tertiary structure, which is influenced by its amino acid sequence. A total 57 WRKY TF were identified in the plant. Biocomputational approaches used to analyze the physicochemical properties of WRKY transcription factor proteins of grapes. In order to characterize the physiochemical properties of TFs, six parameters have been selected: peptide length, Aromaticity, GRAVY, isoelectric point (pI), Molecular weight, and Instability Index. The physiochemical properties of peptide sequences open a new strategy for reagent design and also helps to develop a bioinformatics tool to guide peptide synthesis.

尽管转录因子TF的功能由其三级结构决定，而该结构又受其氨基酸序列的影响，植物中共鉴定出57个WRKY转录因子。为了分析葡萄WRKY转录因子蛋白的物理化学性质，采用了生物计算方法。为了表征转录因子的物理化学性质，选定了六个参数：肽链长度、芳香性、GRAVY、等电点（pI）、分子量和不稳定性指数。肽链序列的物理化学性质为试剂设计开辟了新的策略，同时也助力于开发生物信息学工具，以指导肽链的合成。