Effect of KO of CCND1, ORAOV1, or miR548K in FaDu cancer cell lines or oral keratinocytes. Effect of KO of CCND1, ORAOV1, or miR548K in FaDu cancer cell lines or oral keratinocytes

To investigate the function of CCND1/CCND2/ORAOV1/miR548K in 11q13-amplified cancer cells or non cancerous keratinocytes, we established respective KO cells through CRISPR-Cas9. We performed gene expression profiling analysis using data obtained from RNA-seq upon target KO in either FaDu cells or TP53/CDKN2A k.o. oral keratinocytes Overall design: Comparative gene expression profiling analysis of RNA-seq data for FaDu and TP53/CDKN2A KO oral keratinocytes KO derivatives (CCND1, CCND2, ORAOV1, miR548K, compared to 11q13-noncoding to control for copy number cuts).