Transcriptome-wide N6-methyladenosine modification profiling in patients with recurrent implantation failure (RIF)

Purpose:using m6A modified RNA immunoprecipitation sequence (m6A-seq), to establish the m6A methylation transcription profiles in recurrent implantation failure (RIF). Methods: GenSeq® Low Input Whole RNA Library Prep Kit (GenSeq, Inc.) was used to construct RNA libraries for IP and input samples by following the manufacturer's instructions. The library quality was evaluated using Agilent 2100 bioanalyzer and then sequenced in a NovaSeq platform (Illumina 6000). Result:using m6A modified RNA immunoprecipitation sequence (m6A-seq), methylated sites in mRNA,lncRNA and circRNA are identified. Conclusions: Our study revealed the m6A methylation landscape by MERIP sequencing. Comparative gene expression profiling analysis of MeRIP-seq data for middle secretory phase endometrium in recurrent implantation failure and control. (subjects referred to women, who were pregnant in the next cycle after sampling, underwent IVF/ICSI-ET due to fallopian tube or male factors, and less than 2 fresh or frozen embryos were transferred.)