Transcriptomic profiles of OASL knockdown HUVECs under basal or activated condition

Purpose: The goal of this study is to compare endothelial transcriptome affected by OASL knockdown under basal or stimulated conditions by utilizing RNA-seq. Methods: Endothelial mRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. Following an optimized data analysis workflow, RNA sequence reads were counted using StringTie-2.1.3b and used to identify genes differentially expressed between siCTL- and siOASL-transfected HUVECs treated with or without TNFα and IFNγ. Results: DESeq2 was used to identify genes differentially expressed between siCTL- and siOASL-transfected HUVECs under basal or stimulated conditions. Genes showing <10 read counts total were pre-filtered. Conclusions: Our study represents the first analysis of endothelial mRNA affected by OASL knockdown with biologic replicates, generated by RNA-seq. HUVECs were cultured in EGM-2 BulletKit medium containing 10% FBS, 2mM L-glutamine, and penicillin/streptomycin and used at passage 7-10 for experiments. Two replicates of siRNA-transfected HUVECs per group were treated with or without TNFα and IFNγ added in serum-free EGM-2 for 3hours. Endothelial mRNA profiles of siCTL- and siOASL-transfected HUVECs were generated by RNA-seq.