Table 1 in Morphological and phylogenetic analyses reveal two new species and a new record of Apiospora (Amphisphaeriales, Apiosporaceae) in China
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Table 1. Gene regions and respective primer pairs used in the study.
LocusPCR primersPCR: thermal cycles: (Annealing temperature in bold)ReferenceITSITS5/ITS4(94 °C: 30 s, 55 °C: 30 s, 72 °C: 45 s) × 29 cyclesWhite et al. 1990LSULR0R/LR5(94 °C: 30 s, 48 °C: 50 s, 72 °C: 1 min 30 s) × 35 cyclesVilgalys and Hester 1990; Cubeta et al. 1991tef1EF1-728F/EF2(95 °C: 30 s, 51 °C: 30 s, 72 °C: 1 min) × 35 cyclesO’Donnell et al. 1998; Carbone and Kohn 1999tub2Bt-2a/Bt-2b(95 °C: 30 s, 56 °C: 30 s, 72 °C: 1 min) × 35 cyclesGlass and Donaldson 1995
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2025-04-04



