Stranded RNA-Seq of Arabidopsis thaliana 4 days old seddlings shoot and root tissue 30 minutes and 8 hours after WIP2/NTT induction. Stranded RNA-Seq of Arabidopsis thaliana WIP2/NTT induction lines
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4 days after germination, 35S::NTT-GR (Crawford et al. 2015) seedlings were induced and shoot and root tissue was colected 30 minutes and 8 hours after induction. 30 minutes treatments: 10 uM dexamethasone dissolved in ethanol plus 30 uM cycloheximide (induction) or ethanol (same volume) and 30 uM cycloheximide (control). 8 hours treatments: 100 uM dexamethasone dissolved in ethanol (induction) or ethanol (same volume; control). RNA-Seq libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E. Sequencing was done on an Illumina NovaSeq, 100 bases paired-end.
创建时间：
2022-09-01
