Comparison of the root tip transcriptome of 2 pearl millet lines with contrasted root soil aggregation

We analyzed global gene expression in the crown tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 220) root soil aggregation using RNAseq. The obtective was to identify genes potentially associated with changes in rhizosheath formation. Two pearl millet lines with contrasted root soil aggregation were grown in hydropony for 15 days. 3 biological replicates were produced per line. RNA was extracted from the crown root tip (2 cm apex). RNA-seq was performed by the Montpellier GenomiX Platform (MGX, https://www.mgx.cnrs.fr/). Sequencing was performed on an Illumina HiSeq 2500.