Optimisation of the self-transcribing active regulatory region sequencing massively parallel reporter assay for the functional interrogation of cis-regulatory regions

H3K27ac chromatin was captured and cloned into a modified STARR-seq vector. The captured CREs were assayed for regulatory activity. HEK293T cells were used to capture H3K27ac enriched genomic regions using ChIP-seq and then transfected with the library to measure CRE activity.