Disruption of the MSL complex inhibits tumor maintenance by exacerbating chromosomal instability

Identification of gene expression changes induced after loss of a functional MSL complex in Transformed Dermal Fibroblast cells. Identification of copy number changes induced after loss of a functional MSL complex in Transformed Dermal Fibroblast cells. Overall design: RNA-seq analysis of Transformed Dermal Fibroblast cells transduced with 4-7sgRNAs against members of the MSL complex (MSL1, MSL2, MSL3), control gene (GFP) or non-transduced (WT), and treated with doxycycline to induce Cas9 expression and target gene KO. RNA was collected 14 days post-induction. Grouped samples were compared: MSL KO (MSL1, MSL2 and MSL3 KO) and control (GFP KO, WT). DNA-seq analysis of Transformed Dermal Fibroblast cells transduced with sgRNAs against MSL3, GFP as a control gene (cntr-KO) or non-transduced (WT), and treated with doxycycline to induce Cas9 expression and target gene KO. RNA was collected 14 days post-induction. Grouped samples were compared: MSL KO (MSL1, MSL2 and MSL3 KO) and control (GFP KO, WT).