RNA-Seq Analysis of U21 Treated Toxoplasma gondii Tachyzoites

To assess how the N,N di-aryl urea compound U21 effects Toxoplasma gondii tachyzoties, differential expression analysis via RNA-seq was utilized. Significantly upregulated and downregulated transcripts were submitted for gene ontology analysis for effected biological processes. Briefly, raw reads were aligned to the TgGT1 transcriptome for non-host reads and HG38 for host reads by BowTie2. Quantification of T. gondii transcripts was performed with Salmon and differential expression (Log2 fold change) was calculated with DESeq2. Fold change was gated to a Log2 fold change of 1.5 with a p value less than 0.05. Both upregulated and downregulated transcripts were then inputted into ToxoDB for gene ontology analysis for affected biological processes.