Sedimentary DNA of a human‐impacted lake in Western Canada (Cultus Lake) reveals changes in micro‐eukaryotic diversity over the past ~200 years

Although the use of genetic analyses of sedimentary DNA to track changes in biodiversity has increased over the last decade, questions remain as to how well DNA captures past ecological conditions. Even less is known about how extracellular and intracellular DNA are archived in lake sediments and whether the two fractions yield similar information. Here we characterized the changes of micro‐eukaryotic communities over the past ~200 years in Cultus Lake (British Columbia, Canada), for which a rich body of limnological data and a pre‐existing multi‐proxy paleolimnological study exist. We generated and analyzed 18S rRNA gene amplicons and found that extracellular and intracellular DNA provided different insights, with the preservation of extracellular DNA compromised in sediments older than ~30 years. Principal Coordinates and indicator species analyses based on intracellular DNA showed that changes in micro‐eukaryotic diversity occurred at similar time periods as those identified with the..., Intracellular and extracellular DNA was extracted from sediment samples using the NucleoSpin® Soil kit according to the manufacturer instructions (Macherey-Nagel, Düren, Germany). Beforehand, a phosphate buffer was used to de-adsord the extracellular DNA fraction from sediment particles and the first steps of the commercial DNA extraction kit involving lysis were skipped to avoid further degradation of the extracellular DNA. PCR amplification were performed to amplify the V7 region of the 18S rRNA gene and the samples were sent to Genome Quebec for library preparation and paired-end (2 x 250bp) sequencing on a MiSeq Illumina instrument. The MiSeq reads were then trimmed and filtered, and the paired-end reads were merged with the package dada2 in R software. Taxonomic assignment was performed using the Protist Ribosomal Reference database (PR2)  - SSU rRNA gene database. For more details about the methods, refer to Gauthier et al. (2022; doi: 10.1002/edn3.310)., , # Sedimentary DNA of a human‐impacted lake in Western Canada (Cultus Lake) reveals changes in micro‐eukaryotic diversity over the past \~200 years

## Description of the data and file structure

This following data files were submitted:

1\) SampleDescription_CultusLakeSedimentCore.csv: a sample description with subsample of sediments and age estimation

2\) TaxonomyTable_CultusLakeSedimentCore.csv: the taxonomy of the ASV identified with the 18S rRNA gene primers

3\) V7-18SrRNAsequences_CultusLakeSedimentCore.fasta: the fasta file with the DNA sequence of each ASV

4\) ASVTable_CultusLakeSedimentCore.csv: the number of sequences of the ASVs in each sample

**SampleDescription_CultusLakeSedimentCore.csv**

*SampleID*: Number given to the sample for its location in the core. The A means that it was the A replicate analysed for metabarcoding (collected A and B replicates), and S or P means that it is extracellular (supernatant) or intracellular (pellet). S and P were used because of the ...