Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress

Jasmonic acid (JA) is an endogenous phytohormone that
regulates
plant physiological metabolism and stress response processes, either
independently or through hormone crosstalk. Our phytohormone assay
and transcriptome–metabolome analysis revealed the key genes
and metabolites involved in the JA pathway in response to 0–250
μM cadmium (Cd) in potato seedlings. Transcriptome gene set
enrichment and gene ontology analysis indicated that JA-related genes
were significantly enriched. Specifically, members from the StOPR and StJAZ gene families showed pronounced
responses to Cd stress and methyl jasmonate treatment. As a negative
regulatory transcription factor of the JA signaling pathway, StJAZ14 exhibited a decreasing trend under Cd stress. Yeast
two-hybrid assay identified an interaction between StJAZ14 and StBZR1,
which is located on the brassinolide pathway. In addition to unveiling
the critical role of the JA pathway in regulating potato response
to Cd stress, the functional mechanism was preliminarily explored.