RNA-seq on periodontal tisuues of susceptible and resistant collaborative cross mice after periodontal infection

We assessed alveolar bone changes of Collaborative cross (CC) lines in response to a mixed oral infection with a well-known periodontal pathogen. The CC lines showed significant variation in their response to the mixed infection. We then carried out RNA-seq analysis on periodontal tisuues of resistant CC lines, susceptible CC lines and two CC lines that showed bone formation after oral bacterial infection. We assessed alveolar bone changes of Collaborative cross lines after periodontal infection with P.g. (strain 381) and F.n. (strain PK1594) using micro–computed tomography (µCT) (control groups were treated with PBS and 2% Carboxymethycellulose). The CC lines showed significant variation in their response to the mixed infection. We then carried out RNA-seq analysis on periodontal tisuues of two resistant CC lines (TAU-IL188, TAU-IL111), two susceptible CC lines (TAU-IL785, TAU-IL551) and two CC lines that showed bone formation after oral bacterial infection (TAU-IL2124, TAU-IL2126).