Translatomes of monocytes and macrophages

RNA modifications are essential for the establishment of cellular identity. Although increasing evidence indicates that RNA modifications control the innate immune response, their role in monocyte-to-macrophage differentiation and polarisation is unclear. We profile translatomes of monocytes and macrophages at resting, pro- and anti-inflammatory states. Polyribo-Seq sample preparation and uHPLC Size Exclusion Chromatography (SEC) were performed as described by Yoshikawa et al.,.