Supplemental material Glycolysis and hexosamine biosynthesis pathways in valve cells

(1) Western blot assays for the immunodetection of proteins, i.e., adhesion molecules ICAM-1 and VCAM-1. Whole cell lysates were analyzed by separated by SDS-PAGE, 10% gels. Blots were incubated with primary antibodies recognizing the human form of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1. Data was normalized using antibodies recognizing the housekeeper gene β-tubulin. Densitometer FX and Quantity one software was used to acquire densitometry data for each blot. Images were exported to TIFF and the analysis of the band of interest was performed using Fiji software. Densitometry data were presented as arbitrary units normalized to β-tubulin. Data refer to the indicated biological replicates.