rCRUX Generated MiDeca Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: MiDeca<br> Gene: 16S<br> Length of Target: 154-184<br> get_seeds_local() minimum length: 105<br> get_seeds_local() maximum length: 230<br> blast_seeds() minimum length: 66<br> blast_seeds() maximum length: 191<br> max_to_blast: 50<br> Forward Sequence (5'-3'): GGACGATAAGACCCTATAAA<br> Reverse Sequence (5'-3'): ACGCTGTTATCCCTAAAGT<br> Reference: Komai, T., Gotoh, R.O., Sado, T. and Miya, M., 2019. Development of a new set of PCR primers for eDNA metabarcoding decapod crustaceans. Metabarcoding and Metagenomics, 3, p.e33835. https://doi.org/10.3897/mbmg.6.76534 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.