Comparative assessment of the transcriptome of pancreatic islets from Non-Obese Diabetic mice in the absence of immune infiltrate

The transcriptome of pancreatic islets from four mouse strains was compared - NODk.RagKO, B10k.RagKO, NODk.RagKO.insHEL and B10k.RagKO.insHEL. This comparison allows the assessment of altered transcriptome due to two factors - genetic background of NOD vs B10 strain, and the effect of the insHEL transgene. The use of the MHC k haplotype and the RagKO background remove contamination from infiltrating immune cells, in order to obtain a pure islet transcriptome. Three mice of each gentoype were analyzed in this study.本研究对四种小鼠品系的胰岛（pancreatic islets）转录组（transcriptome）开展比较分析，所涉品系包括NODk.RagKO、B10k.RagKO、NODk.RagKO.insHEL及B10k.RagKO.insHEL。该比较分析可用于评估两种因素引发的转录组改变：一是NOD与B10品系的遗传背景差异，二是insHEL转基因（insHEL transgene）的效应。本研究采用MHC k单倍型（MHC k haplotype）与RagKO背景，以清除浸润免疫细胞带来的污染，从而获得纯净的胰岛转录组。本研究对每种基因型的三只小鼠进行了分析。