Organogenetic transcriptomes of the Drosophila embryo at single cell resolution

To gain insight into the transcription programs activated during organogenesis of Drosophila larval structures, we carried out single cell RNA sequencing during two periods of Drosophila embryogenesis: stages 10 – 12, when most organs are first specified and initiate morphological and physiological specialization, and stages 13 – 16, when organs have achieved their final mature architectures and are beginning to function. Overall design: Two sets of biological replicates for stage 10-12 wild type (Oregon R) Drosophila melanogaster embryos and two sets of biological replicates for stage 13-16 wild type (Oregon R) Drosophila melanogaster embryos were collected, dissociated into single cells, and ran through 10X scRNA-seq pipeline.