Suppression of nucleotidyl transferases affects overall miRNA abundance.

We profiled miRNA expression with the NanoString nCounter platform (left) and miRNA qRT-PCR assays (right) following suppression of a panel of nucleotidyl transferases. Suppression of TUT1, shown in bold, led to the most substantial decreases in miRNA expression compared to cells treated with negative control siRNAs (t-test adjusted p-value <1×10−10 for both NanoString and qRT-PCR platforms). The table depicts the mean and median relative expression values (RQ) of miRNAs robustly detected across all samples. This included 65 miRNAs from NanoString profiling and 217 miRNAs from qRT-PCR arrays.