Supplemental Material for Buzby et al., 2025

Table S1. Variation in most traits of interest is determined by multiple genetic and environmental factors. These influences may combine in complicated ways, for example when the effect of one genetic variant depends on others in the genome. Such dependencies are difficult to identify because testing many possible combinations reduces statistical power. This study combines bulk segregant analysis, a robust method for detecting individual-variant effects, with chromosome fixation in budding yeast. This approach enables detection of variant effects that depend on the fixed chromosome with high power, in addition to the effects not dependent on that fixed chromosome.