scRNA-seq of pancreatic islets from adult male mice with Fltp-reporter sorting and pancreatic head-tail separation

Single-cell RNA sequencing of pancreatic islets from adult male mice. To disect islet heterogeneity we sorted cells based on anatomical location and maturity marker (Fltp). We used the Fltp reporter mouse line FltpZV (Gegg, et al., 2014; https://doi.org/10.7554/elife.03842) in male mice with the age of 4 months. Pancreas head and tail were anatomically separated prior islet isolation. Islets from six FltpZV/+ mice were pooled. Fltp Venus reporter positive and negative cells were sorted. Four libraries were generated based on anatomical region and cell sorting.