Estimated costs of laboratory-based LAMP-CRISPR and qPCR assays.

Per-reaction cost estimates (USD) for LAMP-CRISPR and qPCR assays were calculated based on four categories: assay reagents, sample preparation, disposable materials, and instrument costs. Prices from local distributors included import-related costs applicable in Peru. Instrument costs were estimated using straight-line depreciation (10-year useful life; 10% salvage value), assuming 10,000 reactions per year, following Gutierrez Guarnizo et al. [56]. Recombinant LbCas12a production costs were estimated using the reagent information reported by Mendoza-Rojas et al. [67] and the measured enzyme concentration obtained per liter of E. coli culture (2.2 µM). LAMP-CRISPR runs included 10 clinical samples plus controls. qPCR runs included 12 samples analyzed in triplicate, along with a standard curve and controls. Per-reaction costs were converted to per-sample costs by prorating controls and accounting for technical replicates. (XLSX)