Details of the experimental protocol as applied to the individual sheep used in the present study.

The lung segment nomenclature is as follows: RA – Right apical; RC – Right cardiac; RVD2 and RCD – Right ventral diaphragmatic 2 and Right caudal diaphragmatic (corresponding to the lateral and dorsal basal segments of the right diaphragmatic lobe respectively); LC – Left cardiac (corresponding to the cardiac segment of the apico-cardiac lobe); LVD2 and LCD – Left ventral diaphragmatic 2 and Left caudal diaphragmatic (corresponding to the lateral and dorsal basal segments of the diaphragmatic lobe respectively). The detailed specification of the lung instillates are as described in the materials and methods. Assessment techniques employed included bronchial brush biopsy (BBr) and bronchoalveolar lavage (BAL). During post mortem examination (PME) some segments were inflation fixed (Infl.fixed) with 10% formaldehyde to facilitate histological assessment, or 3% glutaraldehyde in 0.1 M Sodium cacodylate buffer for electron microscopy.