Kellenberger_etal_overdominance_other_datasets

Study:Emergence of a floral colour polymorphism by pollinator-mediated overdominance

Authors:Roman T. Kellenberger, Kelsey J.R.P. Byers, Rita M. De Brito Francisco, Yannick M. Städler, Amy M. LaFountain, Jürg Schönenberger, Florian P. Schiestl, Philipp M. Schlüter

Small datasets:

Table containing
estimated absolute and relative frequencies of G.
rhellicani colour morphs at Puflatsch 1997-2016

Table containing
morphological and fitness data for the G. rhellicani colour morphs in the 14 plots at Puflatsch 2015-2016

Table containing
morphological, metabolomic, and genetic data for the focal G.
rhellicani plants at Puflatsch

Table
containing results of the pollinator exclusion experiment conducted at
Ofenpass in 2016

Table containing
time-lapse camera recordings of insect landings on G.
rhellicani inflorescences at Puflatsch 2016-2017

Table containing
genetic information of the G. rhellicani plants used for time-lapse recordings of insect landings in
2017

Table
containing temperature measurements of G. rhellicani inflorescences at Puflatsch in 2016

Table containing
floral scent profiles from 3 time points, morphological, and genetic
information of G. rhellicani plants

Table containing
morphological, metabolomic, and genetic data for G.
rhellicani plants at Bondone
Table containing raw HPLC-MS/MS data of carotenoid contents in G. rhellicani flowers from Puflatsch and Bondone

Table
containing genetic information of G. rhellicani plants from 13 other Alpine populations

Table
containing qRT-PCR quantifications of GrMYB1 and GrANS1 in
G. rhellicani plants
from Ofenpass treated with GrMYB1 dsRNA

Images focal plants:Compressed folder containing jpeg images of inflorescences of the focal G. rhellicani plants (Puflatsch and Bondone). See small datasets for sample information.

Images plot plants 2015:Compressed folder containing jpeg images of inflorescences of the G. rhellicani plants in the 14 plots established along the Northern ridge of the Puflatsch population in 2015. Brightness of the photos has been increased to facilitate flower counting. Images for plot C are missing. See small datasets for sample information.

Images plot plants fruits 2015:Compressed folder containing jpeg images of infructescences of the G. rhellicani plants in the 14 plots established along the Northern ridge of the Puflatsch population in 2015. Brightness of the photos has been increased to facilitate fruit counting. See small datasets for sample information.

Images plot plants seeds 2015:Compressed folder containing jpeg microscopy images of seeds of the G. rhellicani plants in the 14 plots established along the Northern ridge of the Puflatsch population in 2015. Filename structure: plant ID - date - image number. See small datasets for sample information.

Images plot plants 2016:Compressed folder containing jpeg images of inflorescences of the G. rhellicani plants in the 14 plots established along the Northern ridge of the Puflatsch population re-flowering in 2016. See small datasets for sample information.

Images pollinator exclusion plants fruits 2016:Compressed folder containing jpeg images of infructescences of the G. rhellicani plants used in the pollinator exclusion experiment in the Ofenpass population in 2016. See small datasets for sample information.

Images pollinator exclusion plants seeds 2016:Compressed folder containing jpeg microscopy images of seeds of the G. rhellicani plants used in the pollinator exclusion experiment in the Ofenpass population in 2016. Filename structure: plant ID - date - image number. See small datasets for sample information.

Pollinator recordings 2016 1-3:gzip compressed folders containing part 1, 2, and 3 of the pollinator recordings at the Puflatsch population in 2016
md5 part1 (zip): 29b3bdd842268c5141fb248c4f8bc8be
md5 part2 (zip): 40037e1bd330bb34b00f862813f57f26
md5 part3 (zip): 30ab5a7c5e25225769bed4fc31a1234f

Pollinator recordings 2017 1-2:gzip compressed folders containing part 1 and 2 of the pollinator recordings at the Puflatsch population in 2017
md5 part1 (zip): 21cbe67199a9b06c8c5071dc5c75d2e9md5 part2 (zip): 28a888155319d4fb469b1d6ac52823a2

GC-MS dataset:gzip-compressed files containing GC-MS chromatograms of Gymnadenia rhellicani flowers in MS-format.
md5 (zip): 086b9c85c329ec0713a0db226a95590a
Subfolders:Focal plants Puflatsch 2014 (NrRTK3 and NrRTK4)
Focal plants Bondone 2015 (NrRTK10)Time course plants 2015 (NrRTK4 at 10:00, 15:30, and 21:00)

RSEM dataset:gzip compressed table containing RSEM-generated expression values per transcript and Gymnadenia rhellicani plant individual extracted from the corresponding Lexogen QuantSeq dataset. See small datasets for sample information.

md5 (zip):4ea420187da3f9dcbbd63b98b45962ab

md5 (uncompressed): 5a60c6ec4de1973eec2b929d4ce4de21

TWAS dataset:gzip-compressed vcf file containing sequence variation data per transcript and Gymnadenia plant individual extracted from the corresponding Lexogen QuantSeq dataset.
md5 (zip):
fa9f89cfd7cd8062558893a00df451a8

md5 (uncompressed): 8081a0b6ab1901c2f8ce6ac73ab4ef75
Sample species:GdKB: Gymnadenia conopseaNrRTK: Gymnadenia rhellicani
Sample origin / population:GdKB1: Davos, SwitzerlandGdKB2: Preda, Switzerland
GdKB3: Tschierv, Switzerland
NrRTK3 and NrRTK4: Puflatsch, ItalyNrRTK5: Chandolin, SwitzerlandNrRTK9: Ofenpass, Switzerland

TRINITY dataset:gzip-compressed fasta file containing the Trinity-assembled combined reference transcriptome for Gymnadenia.

md5 (zip):bc1d625b2518f1cb3dc72fb97d87fc07

md5 (uncompressed):43c7838a0ac20c4c23917d6bd4818f32

Gymnadenia samples used for assembly:NrRTK4G - G. rhellicani (Puflatsch, Italy, black)
NrRTK3E2 - G. rhellicani (Puflatsch, Italy, red)NrRTK3F2 - G. rhellicani (Puflatsch, Italy, white)NrRTK5W - G. rhellicani (Chandolin, Switzerland, yellow)NrRTK5X - G. rhellicani (Chandolin, Switzerland, black)
NrRTK9L - G. rhellicani (Ofenpass, Switzerland, black)
GdKB1P - G. densiflora (Davos, Switzerland)GdKB2E - G. densiflora (Preda, Switzerland)
GcKG13 - G. conopsea (Tschierv, Switzerland)GoKG19 - G. odoratissima (Tschierv, Switzerland)

Sequences GrMYB1:gzip-compressed folder containing .ab1 Sanger sequences produced for GrMYB1 genotyping. Filename structure: sample ID - primer - date - well. See small datasets for sample information.

Sequences GrANS1:gzip-compressed folder containing .ab1 Sanger sequences produced for GrANS1 genotyping. Filename structure: sample ID - primer. Primer 1R covers the upstream (reverse complement), and primer 2F covers the downstream part of the cds. See small datasets for sample information.