RIP-seq Experiment for the Leucine Catabolic Enzyme AUH in Brown Fat

The leucine catabolic enzyme AU RNA binding methylglutaconyl-CoA hydratase (AUH) binds to an ARE of mRNA, a sequence containing a cluster of the pentanucleotide AUUUA. To understand the mechanisms of how AUH functions as an RBP here, we performed RNA immunoprecipitation sequencing (RIP-seq) to detect the mRNAs bound to AUH in brown adipose tissue (BAT).