RNA-seq analysis of primary dermal fibroblasts of Hutchinson-Gilford progeria syndrome.

We identified two HGPS patients with the classical mutation site c.1824C>T. We seperated primary dermal fibroblasts from these patients and one normal child, and performed RNA-seq. By analyzing gene expression level, we identified 5567 common differential exprssion genes of two HGPS patients compared to norml child. We classified these genes into three groups, including common up genes (2243), common down genes (2644) and differential genes (680). We focused on common up and common down genes, and performed GO analysis and KEGG analysis. Our study provides a sight for better understanding HGPS and normal aging. RNA-seq analysis of primary dermal fibroblasts from two HGPS patients and one normal child.