Cerebellar development

The Affymetrix GeneChip Mu11K was used to analyze the gene expression profile in developing mouse cerebellum (two GeneChips per E18, P7, P14, P21, and P56) to assist in the understanding of the genetic basis of cerebellar development in mice. The analysis showed 81.6% (10,321/12,654) of the genes represented on the GeneChip were expressed in the postnatal cerebellum, and among those, 8.7% (897/10,321) were differentially expressed with more than a two-fold change in their maximum and minimum expression levels during the developmental time course. The expression data (mean signal in relative unit) of all of these 897 differentially expressed genes were listed in GSM50(for E18), GSM51(for P7), GSM52(for P14), GSM53(for P21), and GSM54(for P56) as well as our homepage at http://www.brain.riken.go.jp/labs/lm Keywords = mouse cerebellum development Keywords: time-course本研究采用Affymetrix GeneChip Mu11K基因芯片（Affymetrix GeneChip Mu11K）分析发育中小鼠小脑的基因表达谱（gene expression profile），其中E18、P7、P14、P21及P56每个时间点均采用两张基因芯片，以助力解析小鼠小脑发育的遗传基础。分析结果显示，该基因芯片上涵盖的12654个基因中，有81.6%（10321/12654）在小鼠生后小脑内存在表达；其中8.7%（897/10321）为差异表达基因，在整个发育时间进程中，其表达量的最高值与最低值间存在超过两倍的变化幅度。上述897个差异表达基因的表达数据（相对单位下的平均信号强度）已分别收录于GSM50（对应E18时期）、GSM51（对应P7时期）、GSM52（对应P14时期）、GSM53（对应P21时期）及GSM54（对应P56时期），亦可通过本研究团队的主页http://www.brain.riken.go.jp/labs/lm获取。关键词：小鼠小脑发育，时间进程