Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma

Single cell mRNA sequencing was used to investigate alterations in the immune microenvironment during multiple myeloma. In the 5TGM1 murine myeloma model, scRNAseq was performed on bone marrow immune cells sorted from steady state and tumor-bearing C57Bl/6JOlaHsd and C57Bl/KaLwRijHsd mice. Tumor-bearing mice were sacrificed 21 days after injection of 5TGM1 cells. Tumor-bearing C57Bl/6JOlaHsd presented with stable bone marrow myeloma, while C57Bl/KaLwRijHsd had progressive bone marrow myeloma. Next, scRNAseq was performed on bone marrow cells from relapsing myeloma patients and non-tumor controls. Compared to non-tumor controls, we observe an expansion of IFN-responsive NK cells in both tumor-bearing mice and in relapsing myeloma patients.