NLRX1 inhibits MAVS-DDX58 interaction

NLRX1 is a member of nucleotide-binding domain and leucine-rich repeat containing (NLR) protein family. NLRX1 competes with RIG-I for IPS-1 interaction and has been identified as a negative regulator of RLR signaling. NLRX1 resides at the outer mitochondrial membrane where IPS-1 is located and this interaction is mediated by the CARD region of IPS-1 and a putative nucleotide-binding domain (NBD) of NLRX1. This interaction between NLRX1 and IPS-1 prevents the association between RIG-1/MDA5 and IPS-1.

NLRX1蛋白属于核苷酸结合结构域和亮氨酸富集重复结构域（NLR）蛋白家族。NLRX1与RIG-I竞争IPS-1的结合位点，并被鉴定为RLR信号通路的负调控因子。NLRX1位于外线粒体膜上，IPS-1也位于此膜上，两者之间的相互作用由IPS-1的CARD结构域和NLRX1的假定的核苷酸结合结构域（NBD）介导。NLRX1与IPS-1之间的相互作用阻止了RIG-1/MDA5与IPS-1的结合。