Genes Identified from a Combined Proteomic and Transcriptomic Screen for candidates.

Gene names, α-catenin co-IP peptide counts, and EPC mRNA enrichment for each candidate are presented. Enrichment data are shown for genes with EPC enrichment over the whole embryo at that stage if EPC(s) also had higher RPKM than cells at earlier stages. Primers were designed to amplify each target gene sequence from cDNA (S2 Table). Each dsRNA was injected into young adults of the wild-type N2 strain of C. elegans. Embryos laid by injected worms were scored 24 hours after injection, filmed by DIC microscopy, and examined for Gad phenotypes. gdi-1 RNAi resulted in 100% sterility, failing to produce embryos for analysis, as seen previously [28].