Genotype of strains and plasmids used in this study.
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a, b, mating type loci; P, promoter; -, fusion; hygR, hygromycin resistance; bleR, phleomycin resistance; natR, nourseothricin resistance; cbxR, carboxin resistance/, ectopically integrated; otef, constitutive promoter; nar, conditional nitrate reductase promoter; E1, W2, genes of the b mating type locus; LifeAct, first 17 aa of Abp140 dyn2: C-terminal half of the dynein heavy chain; rab5a, small endosomal Rab5-like GTPase; cpy, carboxy-peptidase Y; calS, signal sequence of calreticulin from rabbit, HDEL, endoplasmic reticulum retention signal; gal4s, nuclear localization signal of the GAL-4 DNA binding domain from pC-ACT1 (Clontech); SKL, peroxisomal targeting signal; lga2, putative mitochondrial matrix protein.
创建时间：
2015-12-02
