Quantification of wt PTEN Binding to stBLMs by SPR.

Dissociation constants, Kd, in µM for the binding, at room temperature, of wt PTEN to stBLMs prepared by RSE on SAMs composed of HC18∶βME 70∶30 from lipid solutions of the compositions shown. The lipid chain compositions are dioleoyl (DOPC, DOPS) and that of the natural brain extract for PI(4,5)P2 (mostly stearoyl-arachidonoyl), with the exception of the sample denoted as DPPC∶DPPI(4,5)P2, for which the chain composition on both stBLM components is dipalmitoyl. With the exception of the system denoted as PC∶PS∶PI(4,5)P2 (+chol) which was clearly bimodal, all experiments were evaluated as single component fits.